中性粒細胞在大鼠急性重癥胰腺炎肺損傷中的作用機制

中性粒細胞在大鼠急性重癥胰腺炎肺損傷中的作用機制

作者：徐軍，劉學民，馬清涌，潘承恩

【關鍵詞】 急性病
Experimental study on PMN in acute severe pancreatitis with lung injury
　　【Abstract】 AIM: To study the apoptosis and necrosis of polymorphonuclear neutrophils (PMN) from bronchoalveolar lavage fluid in acute severe pancreatitis (ASP) and to discuss the related mechanism. METHODS: Fortyeight male SD rats were divided into 2 groups (ASP group and control group). ASP model was established by injecting with 35 g/L taurocholic acid into the biliopancreatic duct. Both groups were sacrificed at 3, 6 and 12 h with 8 rats at each time point. At scheduled time, bronchoalveolar lavage fluid was drawn and PMNs were then obtained by gradient density gradient centrifugation method. The ratio of apoptosis and necrosis of PMN was assayed by flow cytometry and LDH as well as index of lung permeability were measured. RESULTS: In ASP group, the survival ratio of PMN increased and apoptosis of PMN was delayed. The level of LDH in bronchoalveolar lavage fluid and the index of lung permeability also increased. CONCLUSION: The delay of PMN apoptosis leads to persistent activation and leakage of toxic contents of PMN, which are closely associated with lung injury
　　【Keywords】 acute disease; pancreatitis; lung/injuries; neutrophils
　　【摘要】 目的： 探討急性重癥胰腺炎(ASP)合并急性肺損傷時肺泡灌洗液中性粒細胞(PMN)凋亡和壞死的發生規律及可能涉及的作用機制. 方法： 選取雄性SD大鼠48只，分為ASP實驗組（24只）、對照組（24只）. 實驗組大鼠穿刺胰膽管并注入35 g/L�；悄懰徕c制作ASP大鼠模型，分別在制模后3, 6, 12 h剖殺，于預定時相取支氣管灌洗液，密度梯度離心分離ＰＭＮ，用流式細胞儀測定PMN凋亡、壞死比例，同時測定乳酸脫氫酶(ＬＤＨ)含量、肺通透指數. 結果： ASP組大鼠肺灌洗液中ＰＭＮ存活細胞比例增加（P

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